Asian Fisheries Society

Development of Microsatellite Markers for the Assessment of Genetic Diversity in Leiopotherapon plumbeus Kner 1864


Microsatellite loci from a Philippine-endemic silver perch, Leiopotherapon plumbeus Kner 1864, were isolated, characterised and used in the development of simple sequence repeat (SSR) markers for genetic diversity analysis. Ten primer pairs were designed from a microsatelliteenriched library and polymorphic markers were used in the diversity assessment of populations from three Philippine lakes (Laguna de Bay, Taal Lake and Sampaloc Lake). Seven primers detected a total of 33 alleles and obtained polymorphism information content (PIC) values ranging from 0.12– 0.75. The overall Ho and uHe values across the populations were 0.247 and 0.494, respectively. Pairwise population FST values showed low but significant differentiation while unweighted pair group method with arithmetic mean (UPGMA) cluster analysis revealed a closer relationship between populations from Taal and Sampaloc lakes. The markers used in this study can be used to jumpstart genetic-based breeding programmes and conservation strategies to save the dwindling population of this species.

Publication Date : 2017-06-30

Volume : 30

Issue : 2

Page : 70-86

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Date 2017/06/30
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