Abstract:

Hepatopancreatocytes from hepatopancreas tissue of the oriental shrimp, Penaeus orientalis Kishinouye were cultured in artificial medium in vitro. The new migrating cells could be observed from blind fragments of hepatopancreas tubules, and new cells also formed a confluent monolayer sheet. One primary culture and four subcultures were set up; some were subcultured for 17 and 28 generations, more than five months in vitro. The subcultured cells and fresh hepatopancreas tissue were studied histilogically. The morphology of embryonic cells from hepatopancreas tissue was similar to that of cells obtained from subcultures which were considered to originate from embryonic cells of hepatopancreas tubular epithelium.

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Date 1990/09/01

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