Identification of Random Amplified Polymorphic DNA (RAPD) Markers in Endemic Yellow Catfish, Horabagrus brachysoma (Gunther 1864)
Abstract:
Random amplified polymorphic DNA (RAPD) analysis was applied to individu-als of Horabagrus brachysoma sampled from three geographic locations of Western Ghat river systems in India. Of the thirty-two 10-mer RAPD primers screened initially, ten were chosen and used in a comparative analysis of H. brachysoma collected from Chalak-kudy, Meenachil and Nethravathi river systems. A total of 124 RAPD fragments were amplified, out of which 49 (39.51%) were found to be shared by individuals of all three river systems. The remaining 75 fragments were found to be polymorphic (60.48%). This confirms the suitability of RAPD markers for the study of population genetic structure in yellow catfish stocks.
Publication Date : 2008-09-14
Volume : 21
Issue : 3
Page : 293-304
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Date 2008/09/14
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